THE LONG EXTRA ARMS OF HUMAN TRANSFER RNA((SER)SEC) AND TRANSFER RNA(SER) FUNCTION AS MAJOR IDENTITY ELEMENTS FOR SERYLATION IN AN ORIENTATION-DEPENDENT, BUT NOT SEQUENCE-SPECIFIC MANNER

被引:82
作者
WU, XQ [1 ]
GROSS, HJ [1 ]
机构
[1] BAYER JULIUS MAXIMILIANS UNIV, INST BIOCHEM, BIOZENTRUM, AM HUBLAND, D-97074 WURZBURG, GERMANY
关键词
D O I
10.1093/nar/21.24.5589
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Selenocysteine tRNA [tRNA(Ser)Sec] is charged with serine by the same seryl-tRNA synthetase (SerRS) as the canonical serine tRNAs. Using site-directed mutagenesis, we have introduced a series of mutations into human tRNA(Ser)Sec and tRNA(Ser) in order to study the identity elements of tRNA(Ser)Sec tor serylation and the effect of the orientation of the extra arm. Our results show that the long extra arm is one of the major identity elements for both tRNA(Ser) and tRNA(Ser)Sec and gel retardation assays reveal that it appears to be a prerequisite for binding to the cognate synthetase. The long extra arm functions in an orientation-dependent, but not in a sequence-specific manner. The discriminator base G73 is another important identity element of tRNA(Ser)Sec, whereas the T- and D-arms play a minor role for the serylation efficiency.
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页码:5589 / 5594
页数:6
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