ISOLATION, CHARACTERIZATION, AND CHROMOSOMAL LOCATION OF A GENE ENCODING THE DELTA(1)-PYRROLINE-5-CARBOXYLATE SYNTHETASE IN ARABIDOPSIS-THALIANA

被引：162

作者：

SAVOURE, A ^{[1
]}

JAOUA, S ^{[1
]}

HUA, XJ ^{[1
]}

ARDILES, W ^{[1
]}

VANMONTAGU, M ^{[1
]}

VERBRUGGEN, N ^{[1
]}

机构：

[1] STATE UNIV GHENT,GENET LAB,B-9000 GHENT,BELGIUM

来源：

FEBS LETTERS | 1995年 / 372卷 / 01期

关键词：

ARABIDOPSIS THALIANA; PYRROLINE-5-CARBOXYLATE SYNTHETASE; PROLINE; SALT STRESS;

D O I：

10.1016/0014-5793(95)00935-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A full-length cDNA and the corresponding At-PSS gene encoding the first enzyme of the proline biosynthetic pathway, the Delta(1)-pyrroline-5-carboxylate (P5C) synthetase, mere isolated in Arabidopsis thaliana. The At-P5S cDNA encodes a protein of 717 amino acids showing high identity with the P5C synthetase of Vigna aconitifolia. Strong homology is also found at the N-terminus to bacterial and yeast gamma-glutamyl kinase and at the C-terminus to bacterial gamma-glutamyl phosphate reductase, Putative ATP- and NAD(P)H-binding sites are suggested in the At-PSS protein, The transcribed region of the At-PSS gene is 4.8 kb long and contains 20 exons, Southern analysis suggests the presence of only one At-PSS gene in the A, thaliana genome mapped at the bottom of the chromosome two. Expression analysis of At-P5S in different organs reveals abundant At-PSS transcripts in mature flowering plant, Rapid induction of the Af-PSS gene followed by accumulation of proline was observed in NaCl-treated seedlings suggesting that At-P5S is osmoregulated.

引用

页码：13 / 19

页数：7

共 37 条

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