RAPID ENZYMATIC METHOD FOR THE MEASUREMENT OF MANNITOL IN URINE

被引：24

作者：

BLOOD, J

INGLE, AR

ALLISON, N

DAVIES, GR

HILL, PG

机构：

[1] PORTON PROD LTD,SALISBURY,ENGLAND

[2] LONDON HOSP,COLL MED,GASTROINTESTINAL SCI RES UNIT,LONDON E1 1BB,ENGLAND

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1991年 / 28卷

关键词：

INTESTINAL PERMEABILITY; CELIAC DISEASE;

D O I：

10.1177/000456329102800416

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0-12 mmol/L; no significant interference from other sugars or sugar alcohols could be demonstrated. The method is precise (within-batch CV < 1%), rapid and shows excellent recovery of mannitol in spiked samples. Comparison with gas liquid chromatography shows excellent correlation (r = 0.994) between the two methods.

引用

页码：401 / 406

页数：6

共 19 条

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