TRANSFECTION OF CULTURED FISH CELLS RBCF-1 WITH EXOGENOUS ONCOGENE AND THEIR RESISTANCE TO MALIGNANT TRANSFORMATION

被引：9

作者：

HAYASAKA, K

SATO, M

MITANI, H

SHIMA, A

机构：

[1] Laboratory of Radiation Biology, Zoological Institute, Faculty of Science, Bunkyo-ku, Tokyo, 113, 7-3-1, Hongo

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 96卷 / 02期

关键词：

D O I：

10.1016/0305-0491(90)90387-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells. © 1990.

引用

页码：349 / 354

页数：6

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