METHANOL DOES NOT SPECIFICALLY INHIBIT ENDOGENOUS BETA-GLUCURONIDASE (GUS) ACTIVITY

被引：9

作者：

WILKINSON, JE ^{[1
]}

TWELL, D ^{[1
]}

LINDSEY, K ^{[1
]}

机构：

[1] UNIV LEICESTER,DEPT BOT,LEICESTER LE1 7RH,ENGLAND

来源：

PLANT SCIENCE | 1994年 / 97卷 / 01期

关键词：

METHANOL; ENDOGENOUS BETA-GLUCURONIDASE ACTIVITY; TRANSGENIC PLANTS; PLANT GENE EXPRESSION; NICOTIANA TABACUM; GUS A GENE;

D O I：

10.1016/0168-9452(94)90108-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One of the problems associated with the use of the bacterial gus A gene as a reporter is the presence of 'endogenous GUS' activity in some plant species. A recent paper [1] suggests that the addition of methanol to GUS reaction buffer specifically inhibits endogenous GUS activity while enhancing the activity of an introduced gus A gene. The results described in this paper suggest this phenomenon may be explained by the effect methanol has on cell membranes and demonstrate that it does not specifically inhibit endogenous GUS activity.

引用

页码：61 / 67

页数：7

共 11 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

JEFFERSON RA, 1987, EMBO J, V6, P3301

[3] AN IMPROVED ASSAY FOR BETA-GLUCURONIDASE IN TRANSFORMED-CELLS - METHANOL ALMOST COMPLETELY SUPPRESSES A PUTATIVE ENDOGENOUS BETA-GLUCURONIDASE ACTIVITY [J].