FRACTIONATION OF ESCHERICHIA-COLI ISOACCEPTING TRANSFER-RNA SPECIES BY SEPHAROSE-4B COLUMN CHROMATOGRAPHY

被引:11
作者
COLANTUONI, V [1 ]
GUARINI, L [1 ]
CORTESE, R [1 ]
机构
[1] UNIV NAPLES,FAC MED,IST CHIM BIOL 2,I-80134 NAPLES,ITALY
关键词
D O I
10.1016/S0003-2697(79)80146-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We have determined the elution profile on Sepharose 4B chromatographic column ofthe tRNA isoaccepting species of all 20 amino acids from Escherichia coli MRE 600. Further chromatography on a reversed phase column (RPC-5) is sufficient, in some cases, for a complete purification. © 1979 Academic Press, Inc.
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页码:248 / 250
页数:3
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