VARIATION IN THICKNESS OF THE LARGE CRYOSECTIONS CUT FOR WHOLE-BODY AUTORADIOGRAPHY

被引：3

作者：

ITO, T ^{[1
]}

BRILL, AB ^{[1
]}

机构：

[1] UNIV MASSACHUSETTS,MED CTR,DEPT NUCL MED,55 LAKE AVE N,WORCESTER,MA 01655

来源：

APPLIED RADIATION AND ISOTOPES | 1991年 / 42卷 / 02期

关键词：

D O I：

10.1016/0883-2889(91)90072-9

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

A method to assess variation in thickness of the large cryosections for whole-body autoradiography (WBARG) was described, and the degree of intraslice and interslice variations were determined for our cryomicrotome system (LKB PMV-2250). Intraslice variation in thickness of the 180 x 80 mm cryosection was 0.72-0.92-mu-m within the range of section thickness for WBARG (15-50-mu-m), and interslice variation was 0.89-1.21-mu-m. These potential variations in section thickness should be kept in mind whenever working with quantitative WBARG.

引用

页码：187 / &

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