PROTEIN-SEQUENCE COMPARISONS SHOW THAT THE PSEUDOPROTEASES ENCODED BY POXVIRUSES AND CERTAIN RETROVIRUSES BELONG TO THE DEOXYURIDINE TRIPHOSPHATASE FAMILY

被引:213
作者
MCGEOCH, DJ
机构
[1] MRC Virology Unit, Institute of Virology, University of Glasgow, Glasgow G11 5JR, Church Street
关键词
D O I
10.1093/nar/18.14.4105
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Amino acid sequence comparisons show extensive similarities among the deoxyuridine triphosphatases (dUTPases) of Escherichia coli and of herpesviruses, and the 'protease-like' or 'pseudoprotease' sequences encoded by certain retroviruses in the oncovirus and lentivirus families and by poxviruses. These relationships suggest strongly that the 'pseudoproteases' actually are dUTPases, and have not arisen by duplication of an oncovirus protease gene as had been suggested. The herpesvirus dUTPase sequences differ from the others in that they are longer (about 370 residues, against around 140) and one conserved element ('Motif 3') is displaced relative to its position in the other sequences; a model involving internal duplication of the herpesvirus gene can account effectively for these observations. Sequences closely similar to Motif 3 are also found in phosphofructokinases, where they form part of the active site and fructose phosphate binding structure; thus these sequences may represent a class of structural element generally involved in phosphate transfer to and from glycosides © 1990 Oxford University Press.
引用
收藏
页码:4105 / 4110
页数:6
相关论文
共 19 条
  • [1] A SENSITIVE PROCEDURE TO COMPARE AMINO-ACID-SEQUENCES
    ARGOS, P
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1987, 193 (02) : 385 - 396
  • [2] DNA-SEQUENCE AND EXPRESSION OF THE B95-8 EPSTEIN-BARR VIRUS GENOME
    BAER, R
    BANKIER, AT
    BIGGIN, MD
    DEININGER, PL
    FARRELL, PJ
    GIBSON, TJ
    HATFULL, G
    HUDSON, GS
    SATCHWELL, SC
    SEGUIN, C
    TUFFNELL, PS
    BARRELL, BG
    [J]. NATURE, 1984, 310 (5974) : 207 - 211
  • [3] CARADONNA SJ, 1984, J BIOL CHEM, V259, P5459
  • [4] THE COMPLETE DNA-SEQUENCE OF VARICELLA-ZOSTER VIRUS
    DAVISON, AJ
    SCOTT, JE
    [J]. JOURNAL OF GENERAL VIROLOGY, 1986, 67 : 1759 - 1816
  • [5] DEVEREUX J, 1984, NUCLEIC ACIDS RES, V57, P1023
  • [6] STRUCTURE AND CONTROL OF PHOSPHOFRUCTOKINASE FROM BACILLUS-STEAROTHERMOPHILUS
    EVANS, PR
    HUDSON, PJ
    [J]. NATURE, 1979, 279 (5713) : 500 - 504
  • [7] ISOLATION AND CHARACTERIZATION OF HERPES-SIMPLEX VIRUS TYPE-1 MUTANTS WHICH FAIL TO INDUCE DUTPASE ACTIVITY
    FISHER, FB
    PRESTON, VG
    [J]. VIROLOGY, 1986, 148 (01) : 190 - 197
  • [8] PROFILE ANALYSIS - DETECTION OF DISTANTLY RELATED PROTEINS
    GRIBSKOV, M
    MCLACHLAN, AD
    EISENBERG, D
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (13) : 4355 - 4358
  • [9] LAPATTO P, 1989, NATURE, V342, P299
  • [10] NUCLEOTIDE-SEQUENCE OF THE STRUCTURAL GENE FOR DUTPASE OF ESCHERICHIA-COLI K-12
    LUNDBERG, LG
    THORESSON, HO
    KARLSTROM, OH
    NYMAN, PO
    [J]. EMBO JOURNAL, 1983, 2 (06) : 967 - 971