PURIFICATION OF A HIGH-AFFINITY INOSITOL 1,3,4,5-TETRAKISPHOSPHATE RECEPTOR FROM BRAIN

被引：59

作者：

DONIE, F ^{[1
]}

REISER, G ^{[1
]}

机构：

[1] UNIV TUBINGEN, INST PHYSIOL CHEM, HOPPE SEYLER STR 4, W-7400 TUBINGEN 1, GERMANY

来源：

BIOCHEMICAL JOURNAL | 1991年 / 275卷

关键词：

D O I：

10.1042/bj2750453

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ins(1,3,4,5)P4 has been suggested to be involved in cellular Ca2+ signalling. A receptor from pig cerebellar membranes, which binds InsP4 with high affinity and selectivity [Donie & Reiser (1989) FEBS Lett. 254, 155-158], has been solubilized and purified about 20 000-fold by chromatography using CM-cellulose, heparin-agarose and hydroxyapatite. The InsP4 receptor, identified by SDS/PAGE, had an apparent molecular mass of 42 kDa and bound 4.6 nmol of InsP4 per mg of protein, with a dissociation constant of 5.6 nM.

引用

页码：453 / 457

页数：5

共 25 条

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