MOUSE ANTI-MOUSE IGD MONOCLONAL-ANTIBODIES GENERATED IN IGD-DEFICIENT MICE

被引：24

作者：

ROES, J ^{[1
]}

MULLER, W ^{[1
]}

RAJEWSKY, K ^{[1
]}

机构：

[1] UNIV COLOGNE,INST GENET,D-50931 COLOGNE,GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 183卷 / 02期

关键词：

GENE TARGETING; IGD; MONOCLONAL ANTIBODY; AFFINITY; TOLERANCE;

D O I：

10.1016/0022-1759(95)00059-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The production of high-affinity monoclonal antibodies (mABs) is generally restricted to antigens recognised as foreign by the immune system. Here we report the generation of mouse mABs specific for mouse IgD. Mice rendered IgD-deficient by gene targeting and consequently immunologically fully responsive to mouse IgD, were used to elicit a humoral response against mouse IgD. Hybridomas producing mABs of high affinity were isolated and clones specific for non-allotypic determinants on the Fc or Fab portion of mouse IgD were obtained. The data show that mice lacking a protein of interest due to targeted gene inactivation can be utilised for the production of high-affinity mABs specific for that mouse protein and should also facilitate the generation of mABs specific for proteins highly conserved between species.

引用

页码：231 / 237

页数：7

共 19 条

[1] MOUSE IMMUNOGLOBULIN RECEPTORS ON LYMPHOCYTES - IDENTIFICATION OF IGM AND IGD MOLECULES BY TRYPTIC CLEAVAGE AND POSTULATED ROLE FOR CELL-SURFACE IGD [J].