LEUKOTRIENE B-4 PRODUCTION BY BLOOD NEUTROPHILS IN ALLERGIC RHINITIS - EFFECTS OF CETIRIZINE

被引：30

作者：

CHERIASAMMARI, S

ALOUI, R

GORMAND, F

CHABANNES, B

GALLET, H

GROSCLAUDE, M

MELAC, M

RIHOUX, JP

PERRINFAYOLLE, M

LAGARDE, M

PACHECO, Y

机构：

[1] CTR HOSP LYON SUD,INSERM,U352,IMMUNOALLERGOL LAB,F-69310 PIERRE BENITE,FRANCE

[2] LAB UCB PHARMA,BRAINE LALLEUD,BELGIUM

[3] INST NATL SCI APPL,INSERM,U352,VILLEURBANNE,FRANCE

来源：

CLINICAL AND EXPERIMENTAL ALLERGY | 1995年 / 25卷 / 08期

关键词：

ALLERGIC RHINITIS; NEUTROPHIL; LTB(4); GM-CSF; ASTHMA; ANTI-HISTAMINIC DRUGS;

D O I：

10.1111/j.1365-2222.1995.tb00010.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Background Mucosal inflammatory processes in late phase of allergic diseases involve cytokine production, cell adhesion molecule overexpression and release of inflammatory mediators with chemotactic activity, such as leukotriene B-4 (LTB(4)). We had previously observed increased production of LTB(4) by neutrophils in patients with allergic rhinitis and discussed the role of granulocyte macrophage-colony stimulating factor (GM-CSF) priming. Some antihistaminic compounds were shown to diminish the production of leukotrienes by neutrophils. Objective In a first step, we evaluated in ex vivo and in vitro studies, the effects of cetirizine on LTB(4) production by blood neutrophils from allergic and healthy subjects. In a second step, we studied the in vitro effect of cetirizine on LTB(4) production by neutrophils from healthy subjects during GM-CSF priming of these cells. Methods Neutrophils from both populations were purified from venous blood and LTB(4) production was measured using high performance liquid cromatography (HPLC) method. Results In ex vivo studies, cetirizine treatment induced a decreased LTB(4) production by neutrophils in allergic rhinitis. This effect of decreased LTB(4) production was reproduced in vitro with 10(-8)-10(-6) M cetirizine. Nevertheless, this anti-H1 compound had no effect on neutrophil priming with GM-CSF. Conclusion As LTB(4) is an important chemotactic factor, Cetirizine could act on inflammatory cell recruitment by inhibiting LTB(4) production by neutrophils.

引用

页码：729 / 736

页数：8

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