A COMPARISON OF DIFFERENT CULTURE METHODS FOR HYBRIDOMA PROPAGATION AND MONOCLONAL-ANTIBODY PRODUCTION

被引：7

作者：

MARQUIS, CP

HARBOUR, C

BARFORD, JP

LOW, KS

机构：

[1] Department of Chemical Engineering, University of Sydney, 2006, N.S.W.

[2] Department of Infectious Diseases, University of Sydney, 2006, N.S.W.

来源：

CYTOTECHNOLOGY | 1990年 / 4卷 / 01期

关键词：

batch; continuous; hybridoma; monoclonal; perfusion; productivity;

D O I：

10.1007/BF00148812

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A major variable to consider in the production of biologicals from mammalian cell cultures is the mode of operation, be it a batch, continuous, perfusion, fed-batch or other production method. The final choice must consider a number of fundamental and economic issues. Here we present some antibody production data from different cell lines using different modes of production and discuss the important factors for consideration in choosing a production strategy. It was found that the productivity of batch cultures was lower than that obtained in continuous and perfused cultures, but that productivity could be improved by implementing suitable feeding strategies. The antibody productivity of one cell line, MCL1, during exponential phase was not affected by media type or glucose level. The maximum productivity of two cell lines in continuous culture was found to occur at dilution rates below the maximum, from 0.019 to 0.030 hr-1. © 1990 Kluwer Academic Publishers.

引用

页码：69 / 76

页数：8

共 18 条

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