PHYTOCHROME IN LOWER PLANTS - DETECTION AND PARTIAL SEQUENCE OF A PHYTOCHROME GENE IN THE MOSS CERATODON-PURPUREUS USING THE POLYMERASE CHAIN-REACTION

被引：17

作者：

THUMMLER, F

BEETZ, A

RUDIGER, W

机构：

[1] Botanisches Institut der Universität München, 8000 München 19

来源：

FEBS LETTERS | 1990年 / 275卷 / 1-2期

关键词：

Amino acid sequence; DNA sequence; Moss; Phytochrome; Polymerase chain reaction;

D O I：

10.1016/0014-5793(90)81455-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The polymerase chain reaction was carried out with primers hybridizing to conserved regions of the phytochrome genes. With DNA from the moss Ceratodon purpureus 5 overlapping fragments were obtained resulting in a continuous nucleotide sequence of 1474 bp. The deduced amino acid sequence showed homology of around 60% with all known phytochrome sequences. The sequences contained a conserved chromophore attachment site. In light-grown Ceratodon protonemata the phytochrome mRNA with the size of about 4.5 kb was detected. © 1990.

引用

页码：125 / 129

页数：5

共 23 条

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Ausubel FM., 1988, CURRENT PROTOCOLS MO

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