THE 5-55 SINGLE-DISULFIDE INTERMEDIATE IN FOLDING OF BOVINE PANCREATIC TRYPSIN-INHIBITOR

被引：37

作者：

DARBY, NJ

VANMIERLO, CPM

CREIGHTON, TE

机构：

[1] EUROPEAN MOLEC BIOL LAB,POSTFACH 102209,W-6900 HEIDELBERG,GERMANY

[2] MRC,MOLEC BIOL LAB,CAMBRIDGE CB2 2QH,ENGLAND

来源：

FEBS LETTERS | 1991年 / 279卷 / 01期

关键词：

PROTEIN FOLDING; DISULFIDE INTERMEDIATE; BOVINE PANCREATIC TRYPSIN INHIBITOR; NUCLEAR MAGNETIC RESONANCE; CIRCULAR DICHROISM;

D O I：

10.1016/0014-5793(91)80251-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An analogue of the BPTI folding intermediate that contains only the disulphide bond between Cys-5 and Cys-55 has been prepared by mutation of the other four Cys residues to Ser. On the basis of its circular dichroism and H-1-nuclear magnetic resonance spectra and its electrophoretic mobility, this intermediate is shown to be at least partially folded at low temperatures. This probably accounts for several of the unique properties of this intermediate observed during folding.

引用

页码：61 / 64

页数：4

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