CHANGE IN THE EXPRESSION OF A NUCLEAR MATRIX-ASSOCIATED PROTEIN IS CORRELATED WITH CELLULAR-TRANSFORMATION

被引：28

作者：

BRANCOLINI, C

SCHNEIDER, C

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 16期

关键词：

ONCOGENES; CELL GROWTH; SPLICEOSOMES NUCLEAR MATRIX; MONOCLONAL ANTIBODIES;

D O I：

10.1073/pnas.88.16.6936

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have identified a monoclonal antibody that recognizes a nuclear matrix-associated protein in NIH 3T3 cells. The immunofluorescence pattern consists predominantly of bright nuclear granule clusters distributed throughout the nucleoplasm, with the exclusion of nucleoli. It recognizes a protein of 190 kDa that is down-regulated to various degrees in a panel of single-oncogene-transformed NIH 3T3 cells. Its localization is similar, but not identical, to the spliceosomal speckles. p190 shows a coordinate expression during the growth cycle of nontransformed NIH 3T3 cells; it is synthesized at the highest level under growth arrest conditions. It is expressed in adult mouse brain and is also present in human IMR-90 fibroblasts.

引用

页码：6936 / 6940

页数：5

共 29 条

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