DIRECT OPTICAL IMMUNOSENSING (SENSITIVITY AND SELECTIVITY)

被引：62

作者：

HUBER, W ^{[1
]}

BARNER, R ^{[1
]}

FATTINGER, C ^{[1
]}

HUBSCHER, J ^{[1
]}

KOLLER, H ^{[1
]}

MULLER, F ^{[1
]}

SCHLATTER, D ^{[1
]}

LUKOSZ, W ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,OPT LAB,CH-8093 ZURICH,SWITZERLAND

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1992年 / 6卷 / 1-3期

关键词：

D O I：

10.1016/0925-4005(92)80042-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper compares theoretically predicted detection limits of three optical sensing devices (surface plasmon resonance, input grating coupler, difference interferometer) with the experimentally found detection limits for anti h-IgG antibodies. The theoretically predicted sensitivities of these sensors are confirmed satisfactorily by experiments. The influence of high concentrations of non-analyte proteins on direct immunosensing with such optical transducers is investigated in the detection of HBsAg (hepatitis B surface antigen) using undiluted human serum with a sensitized surface plasmon resonance sensor; no serious influence was found.

引用

页码：122 / 126

页数：5

共 6 条

[1] BARNER R, 1991, P SENSORS 91 C NURNB, P83
[2] FATTINGER C, IN PRESS
[3] SURFACE-PLASMON RESONANCE FOR GAS-DETECTION AND BIOSENSING
LIEDBERG, B
NYLANDER, C
LUNDSTROM, I
[J]. SENSORS AND ACTUATORS, 1983, 4 (02): : 299 - 304
[4] PRINCIPLES AND SENSITIVITIES OF INTEGRATED OPTICAL AND SURFACE-PLASMON SENSORS FOR DIRECT AFFINITY SENSING AND IMMUNOSENSING
LUKOSZ, W
[J]. BIOSENSORS & BIOELECTRONICS, 1991, 6 (03) : 215 - 225
[5] LUKOSZ W, 1990, SENSOR ACTUAT A-PHYS, V25, P185
[6] TIEFENTHALER K, 1989, SENSOR ACTUAT B-CHEM, V1, P592

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