RECEPTOR-COUPLED SHORTENING OF ALPHA-TOXIN-PERMEABILIZED SINGLE SMOOTH-MUSCLE CELLS FROM THE GUINEA-PIG STOMACH

被引:18
作者
ONO, T
MITA, M
SUGA, O
HASHIMOTO, T
OISHI, K
UCHIDA, MK
机构
[1] MEIJI COLL PHARM,DEPT MOLEC PHARMACOL,1-35-23 NOZAWA,SETAGAYA KU,TOKYO 154,JAPAN
[2] MEIJI COLL PHARM,DEPT PHARMACOL,SETAGAYA KU,TOKYO 154,JAPAN
关键词
CA2+ SENSITIVITY; PERMEABILIZATION; SMOOTH MUSCLE CELLS; MUSCARINIC RECEPTOR; GTP-BINDING PROTEIN;
D O I
10.1111/j.1476-5381.1992.tb14371.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1 Isolated single smooth muscle cells from the fundus of the guinea-pig stomach were permeabilized by use of Staphylococcus aureus alpha-toxin. Receptor-coupled shortening of individual cells was monitored under phase contrast microscopy. 2 Most of the isolated cells responded to 0.6-mu-M Ca2+, but not to 0.3-mu-M Ca2+ with a resulting maximal shortening to approximately 65% of the resting cell length. The contractile activity of these permeabilized cells lasted for several hours and repeated shortening was readily achieved after washing out. 3 Addition of acetylcholine (ACh) at a maximal concentration (10-mu-M) resulted in a marked decrease in the concentration of Ca2+ required to trigger a threshold response from 0.6-mu-M to 0.2-mu-M, and 1 mM guanosine 5'-diphosphate (GDP) blocked this decrease. Moreover, treatment with 100-mu-M guanosine 5'-triphosphate (GTP) mimicked the action of ACh. 4 Addition of 100-mu-M inositol 1,4,5-trisphosphate (InSP3) with 0.2-mu-M Ca2+ did not cause cell shortening, whereas 10-mu-M ACh with 0.2-mu-M Ca2+ did, suggesting that InsP3-induced Ca2+ release is not involved in ACh-operated cell shortening. 5 The present study demonstrates an alpha-toxin-permeabilized single smooth muscle cell preparation which retains its receptor function and also provides an insight into mechanisms leading to augmentation of Ca2+ sensitivity by stimulation of muscarinic receptors or GTP-binding proteins.
引用
收藏
页码:539 / 543
页数:5
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