CALCIUM-ION BINDING OF 3 DIFFERENT TYPES OF OLIGO/POLYSIALIC ACIDS AS STUDIED BY EQUILIBRIUM DIALYSIS AND CIRCULAR DICHROIC METHODS

被引：47

作者：

SHIMODA, Y

KITAJIMA, K

INOUE, S

INOUE, Y

机构：

[1] UNIV TOKYO,FAC SCI,DEPT BIOPHYS & BIOCHEM,TOKYO 113,JAPAN

[2] SHOWA UNIV,SCH PHARMACEUT SCI,TOKYO 142,JAPAN

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 05期

关键词：

D O I：

10.1021/bi00171a020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ca2+ binding properties of three different types of oligo/polysialic acid chains, i.e., oligo/poly(Neu5Ac), oligo/poly(Neu5Gc), and oligo/poly(KDN), were studied by equilibrium dialysis and circular dichroism. Colominic acid, high molecular weight polysialoglycoprotein (H-PSGP), low molecular weight polysialoglycoprotein (L-PSGP), and 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid (KDN) glycoprotein were found to bind calcium ions with about 8-100 times the affinity of sialic acid monomer. Analysis by equilibrium dialysis of the binding of Ca2+ to colominic acid was biphasic, and the high-affinity interaction was shown to change with the degree of polymerization. Specific binding of Ca2+ to polysialic acid (polySia) caused characteristic effects in the circular dichroism spectrum. A pronounced decrease in the circular dichroism of polySia at 205 nm was observed upon addition of calcium. H-PSGP was found to bind calcium ions with 3-fold higher affinity than L-PSGP.

引用

页码：1202 / 1208

页数：7

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[1] NCAM POLYSIALIC ACID CAN REGULATE BOTH CELL CELL AND CELL SUBSTRATE INTERACTIONS [J].