REGULATION OF QUAIL OVIDUCT PHOSPHOLIPASE A(2) ACTIVITY BY ESTRADIOL

被引：11

作者：

FAYARD, JM ^{[1
]}

CHANAL, S ^{[1
]}

FELOUATI, B ^{[1
]}

MACOVSCHI, O ^{[1
]}

LAGARDE, M ^{[1
]}

PAGEAUX, JF ^{[1
]}

LAUGIER, C ^{[1
]}

机构：

[1] INSERM,U352,CHIM BIOL LAB,VILLEURBANNE,FRANCE

来源：

EUROPEAN JOURNAL OF ENDOCRINOLOGY | 1994年 / 131卷 / 02期

关键词：

D O I：

10.1530/eje.0.1310205

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The phospholipase A(2)(PLA(2)) activity was measured in the oviduct ofimmature and estradiol benzoate (EB)-treated quails. The pH profiles demonstrate the presence of two PLA(2) isoforms in the avian oviduct: a neutral isoform, optimally active at pH 7-7.5 and calcium independent, responsible for most of the hydrolytic activity in the immature oviduct and poorly stimulated by estradiol; and an alkaline isoform, optimally active at pH 8-9.5 and calcium dependent, with little activity in the immature tissue but markedly stimulated by EB. After EB injection, PLA(2) activation occurs at first during the prereplicative period of oviduct cells (+172% at 6h), it is dose dependent from 0.01 to 1 mg/kg EB and can be prevented by cycloheximide together with ornithine decarboxylase activation. Moreover, estradiol was inactive on cell-free extracts of immature oviducts. These results suggest that EB increases PLA(2) activity through gene activation and de novo protein synthesis. The correlation between the early stimulation of PLA(2) activity and the proliferation of oviduct cells is discussed.

引用

页码：205 / 212

页数：8

共 38 条

[1]

ARITA H, 1991, J BIOL CHEM, V266, P19139

[2] OPPOSITE EFFECTS OF GLUCOCORTICOID ON ESTROGEN-INDUCED GROWTH AND DIFFERENTIATION OF QUAIL OVIDUCT - DEMONSTRATION BY SEQUENTIAL TREATMENTS [J].