ADHESION FORCES BETWEEN INDIVIDUAL LIGAND-RECEPTOR PAIRS

被引：1623

作者：

FLORIN, EL ^{[1
]}

MOY, VT ^{[1
]}

GAUB, HE ^{[1
]}

机构：

[1] TECH UNIV MUNICH,DEPT PHYS,D-85748 GARCHING,GERMANY

来源：

SCIENCE | 1994年 / 264卷 / 5157期

关键词：

D O I：

10.1126/science.8153628

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 +/- 20 piconewtons for biotin and 85 +/- 15 piconewtons for iminobiotin. The measured force quanta are interpreted as the unbinding forces of individual molecular pairs.

引用

页码：415 / 417

页数：3

共 24 条

[1] [Anonymous], 1992, INTERMOLECULAR SURFA
[2] OPTICAL TRAPPING AND MANIPULATION OF SINGLE CELLS USING INFRARED-LASER BEAMS
ASHKIN, A
DZIEDZIC, JM
YAMANE, T
[J]. NATURE, 1987, 330 (6150) : 769 - 771
[3] ATOMIC FORCE MICROSCOPE
BINNIG, G
QUATE, CF
GERBER, C
[J]. PHYSICAL REVIEW LETTERS, 1986, 56 (09) : 930 - 933
[4] 2-DIMENSIONAL CRYSTALS OF STREPTAVIDIN ON BIOTINYLATED LIPID LAYERS AND THEIR INTERACTIONS WITH BIOTINYLATED MACROMOLECULES
DARST, SA
AHLERS, M
MELLER, PH
KUBALEK, EW
BLANKENBURG, R
RIBI, HO
RINGSDORF, H
KORNBERG, RD
[J]. BIOPHYSICAL JOURNAL, 1991, 59 (02) : 387 - 396
[5] IMAGING CRYSTALS, POLYMERS, AND PROCESSES IN WATER WITH THE ATOMIC FORCE MICROSCOPE
DRAKE, B
PRATER, CB
WEISENHORN, AL
GOULD, SAC
ALBRECHT, TR
QUATE, CF
CANNELL, DS
HANSMA, HG
HANSMA, PK
[J]. SCIENCE, 1989, 243 (4898) : 1586 - 1589
[6] SYNTHETIC LIPID-ANCHORED RECEPTORS BASED ON THE BINDING-SITE OF A MONOCLONAL-ANTIBODY
EGGER, M
HEYN, SP
GAUB, HE
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1104 (01) : 45 - 54
[7] DETACHMENT OF AGGLUTININ-BONDED RED-BLOOD-CELLS .1. FORCES TO RUPTURE MOLECULAR-POINT ATTACHMENTS
EVANS, E
BERK, D
LEUNG, A
[J]. BIOPHYSICAL JOURNAL, 1991, 59 (04) : 838 - 848
[8] EVANS EH, IN PRESS
[9] ATOMIC-FORCE MICROSCOPE WITH MAGNETIC FORCE MODULATION
FLORIN, EL
RADMACHER, M
FLECK, B
GAUB, HE
[J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 1994, 65 (03) : 639 - 643
[10] FRAUENFELDER H, 1988, ANNU REV BIOPHYS BIO, V17, P451

← 1 2 3 →