CLONING AND CHARACTERIZATION OF 2 UBIQUITIN--79-AMINO-ACID EXTENSION PROTEIN-ENCODING FUSION GENES FROM LUPINUS-ALBUS

被引：4

作者：

JACINTO, A

NEVES, AM

VASSILEVSKAIA, TD

RICARDO, CP

RODRIGUESPOUSADA, C

机构：

[1] GULBENKIAN INST SCI,GENET MOLEC LAB,P-2781 OEIRAS,PORTUGAL

[2] INST TECNOL QUIM & BIOL,BIOQUIM VEGETAL LAB,P-2781 OEIRAS,PORTUGAL

来源：

GENE | 1994年 / 139卷 / 02期

关键词：

GENE FAMILY; RIBOSOMAL PROTEIN; CODON USAGE; NUCLEAR SIGNAL; DICOTYLEDONS; LEGUMES;

D O I：

10.1016/0378-1119(94)90755-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nudeotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.

引用

页码：201 / 205

页数：5

共 22 条

[1]

CALLIS J, 1990, J BIOL CHEM, V265, P12486

[2] SEQUENCE-ANALYSIS AND TRANSCRIPTIONAL REGULATION BY HEAT-SHOCK OF POLYUBIQUITIN TRANSCRIPTS FROM MAIZE [J].