STRUCTURAL-CHANGES OF ULTRAVIOLET-IRRADIATED DNA DERIVED FROM HYDRODYNAMIC MEASUREMENTS

被引：13

作者：

TRIEBEL, H ^{[1
]}

REINERT, KE ^{[1
]}

BAR, H ^{[1
]}

LANG, H ^{[1
]}

机构：

[1] AKAD WISSENSCH DDR, BIOCHEM ABT, DDR-69 JENA, GER DEM REP

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 561卷 / 01期

关键词：

DNA conformation; DNA damage; Ultraviolet irradiation;

D O I：

10.1016/0005-2787(79)90490-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The paper reports on sedimentation and viscosity measurements performed on ultraviolet-irradiated DNA from T7 phage and calf thymus. From the hydrodynamic data the relative changes in the mean molecular weight, radius of gyration, and effective Kuhn statistical segment length were calculated. The results show that ultraviolet irradiation (254 nm) leads to a significant decrease of the effective statistical segment length of DNA which may be due to small local helix kinks (produced by the generation of photodimers) and a local increase of chain flexibility. Alterations in the overall DNA conformation may be observed even at low fluences where the mean molecular weight almost stays constant. The locally distorted helix regions possibly may serve as recognition sites in the first step of excision repair. © 1979.

引用

页码：59 / 68

页数：10

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