ENZYMATIC MECHANISM OF CREATINE AMIDINOHYDROLASE AS DEDUCED FROM CRYSTAL-STRUCTURES

被引:58
作者
COLL, M [1 ]
KNOF, SH [1 ]
OHGA, Y [1 ]
MESSERSCHMIDT, A [1 ]
HUBER, R [1 ]
MOELLERING, H [1 ]
RUSSMANN, L [1 ]
SCHUMACHER, G [1 ]
机构
[1] BOEHRINGER MANNHEIM,PENZBERG,GERMANY
关键词
D O I
10.1016/0022-2836(90)90201-V
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Crystal structures of the enzyme creatine amidinohydrolase (creatinase, EC 3.5.3.3) with two different inhibitors, the reaction product sarcosine and the substrate creatine, bound have been analyzed by X-ray diffraction methods. With the inhibitor carbamoyl sarcosine, two different crystal forms at different pH values have been determined. An enzymatic mechanism is proposed on the basis of the eight structures analyzed. The enzyme binds substrate and inhibitor in a distorted geometry where the urea resonance is broken. His232 is the general base and acid, and acts as a proton shuttle. It withdraws a proton from water 377 and donates it to the N(3) atom of the guanidinium group. OH- 377 adds to the C(1) atom of the guanidinium group to form a urea hydrate. Proton withdrawal by His232 leads to products. The reaction product sarcosine binds to the active site in a reverse orientation. The free enzyme was found to have a bicarbonate bound to the active site. © 1990 Academic Press Limited.
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页码:597 / 610
页数:14
相关论文
共 31 条
[1]   THE PATHWAY OF CREATINE CATABOLISM BY PSEUDOMONAS-OVALIS [J].
APPLEYARD, G ;
WOODS, DD .
JOURNAL OF GENERAL MICROBIOLOGY, 1956, 14 (02) :351-365
[2]   STRUCTURAL AND FUNCTIONAL-ASPECTS OF DOMAIN MOTIONS IN PROTEINS [J].
BENNETT, WS ;
HUBER, R .
CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1984, 15 (04) :291-384
[3]   CRYSTALLOGRAPHIC R-FACTOR REFINEMENT BY MOLECULAR-DYNAMICS [J].
BRUNGER, AT ;
KURIYAN, J ;
KARPLUS, M .
SCIENCE, 1987, 235 (4787) :458-460
[4]  
COLMAN PM, 1975, CRYSTALLOGRAPHIC COM, P248
[5]   A METHOD OF POSITIONING A KNOWN MOLECULE IN AN UNKNOWN CRYSTAL STRUCTURE [J].
CROWTHER, RA ;
BLOW, DM .
ACTA CRYSTALLOGRAPHICA, 1967, 23 :544-&
[6]  
EGLOFF K, 1990, THESIS L MAXIMILIANS
[7]   REFINED CRYSTAL-STRUCTURE OF BOVINE BETA-TRYPSIN AT 1.8 A RESOLUTION .1. CRYSTALLIZATION, DATA-COLLECTION AND APPLICATION OF PATTERSON SEARCH TECHNIQUES [J].
FEHLHAMMER, H ;
BODE, W .
JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (04) :683-692
[8]  
HAHN T, INT TABLES CRYSTALLO, VA
[9]   CRYSTAL-STRUCTURE DETERMINATION, REFINEMENT AND MOLECULAR-MODEL OF CREATINE AMIDINOHYDROLASE FROM PSEUDOMONAS-PUTIDA [J].
HOEFFKEN, HW ;
KNOF, SH ;
BARTLETT, PA ;
HUBER, R ;
MOELLERING, H ;
SCHUMACHER, G .
JOURNAL OF MOLECULAR BIOLOGY, 1988, 204 (02) :417-433
[10]  
HOPPE W, 1957, ACTA CRYSTALLOGR, V10, P750