PROTEIN-BINDING A+T-RICH MOTIFS FLANK THE DUCK BETA-A-GLOBIN ENHANCER

被引：9

作者：

CVEKL, A

HORSKA, K

VLCEK, C

PACES, V

机构：

[1] CZECHOSLOVAK ACAD SCI, INST MOLEC GENET, FLEMINGOVO 2, CS-16637 PRAGUE 6, CZECHOSLOVAKIA

[2] CZECHOSLOVAK ACAD SCI, INST ORGAN CHEM & BIOCHEM, CS-16637 PRAGUE 6, CZECHOSLOVAKIA

来源：

GENE | 1991年 / 103卷 / 02期

关键词：

TRANSCRIPTION FACTORS; FOOTPRINTING; RECOMBINANT DNA; AVIAN GLOBIN-ENCODING GENES;

D O I：

10.1016/0378-1119(91)90282-G

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The duck beta-A-globin (beta-GLB) enhancer DNA was analysed by footprinting for sites of specific binding of proteins extracted from duck erythrocytes. The results were compared with previously determined protein binding to the homologous region in chicken DNA. Two A + T-rich protein-binding sites, not recognized in chicken, were found at the 5'-end and the 3'-end of the duck beta-GLB enhancer. The 5'-motif (designated BS-1; 5'-AAACAAAATGAA) binds proteins extracted from both embryonic and adult erythrocytes, while the 3'-motif (BS-2; 5'-ATAAACAAGGTC) binds protein from embryonic cells only.

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页码：253 / 257

页数：5

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