A PROMOTER TRAP IN EMBRYONIC STEM (ES) CELLS SELECTS FOR INTEGRATION OF DNA INTO CPG ISLANDS

被引：20

作者：

MACLEOD, D ^{[1
]}

LOVELLBADGE, R ^{[1
]}

JONES, S ^{[1
]}

JACKSON, I ^{[1
]}

机构：

[1] NATL INST MED RES,MRC,EUKARYOT MOLEC BIOL LAB,LONDON NW7 1AA,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/nar/19.1.17

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An analysis of several G418-resistant ES cell lines produced by electroporation of a promoterless neo(R) gene (NASTI), shows an enrichment for integrations within, or adjacent to, CpG islands. A detailed analysis of two of the cell lines reveals short regions of homology between the genomic target DNA and the construct ends, and that recombination may be mediated by DNA Topoisomerase I. The DNA flanking the insert detects transcription of endogenous genes, and in one cell line divergent transcripts are detected. This use of ES cells should provide an effective and efficient means of creating insertional mutations in mice.

引用

页码：17 / 23

页数：7

共 53 条

[1] TRANSGENES AS PROBES FOR ACTIVE CHROMOSOMAL DOMAINS IN MOUSE DEVELOPMENT
ALLEN, ND
CRAN, DG
BARTON, SC
HETTLE, S
REIK, W
SURANI, MA
[J]. NATURE, 1988, 333 (6176) : 852 - 855
[2] FUNCTIONAL CLONING OF MOUSE CHROMOSOMAL LOCI SPECIFICALLY ACTIVE IN EMBRYONAL CARCINOMA STEM-CELLS
BHAT, K
MCBURNEY, MW
HAMADA, H
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (08) : 3251 - 3259
[3] 2 CLASSES OF OBSERVED FREQUENCY FOR RARE-CUTTER SITES IN CPG ISLANDS
BIRD, AP
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (22) : 9485 - 9485
[4] CPG ISLANDS AS GENE MARKERS IN THE VERTEBRATE NUCLEUS
BIRD, AP
[J]. TRENDS IN GENETICS, 1987, 3 (12) : 342 - 347
[5] CPG-RICH ISLANDS AND THE FUNCTION OF DNA METHYLATION
BIRD, AP
[J]. NATURE, 1986, 321 (6067) : 209 - 213
[6] WEIGHT MATRIX DESCRIPTIONS OF 4 EUKARYOTIC RNA POLYMERASE-II PROMOTER ELEMENTS DERIVED FROM 502 UNRELATED PROMOTER SEQUENCES
BUCHER, P
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 212 (04) : 563 - 578
[7] ASSOCIATION OF CROSSOVER POINTS WITH TOPOISOMERASE-I CLEAVAGE SITES - A MODEL FOR NONHOMOLOGOUS RECOMBINATION
BULLOCK, P
CHAMPOUX, JJ
BOTCHAN, M
[J]. SCIENCE, 1985, 230 (4728) : 954 - 958
[8] ISOLATION OF LARGE DNA FRAGMENTS FROM AGAROSE GELS USING AGARASE
BURMEISTER, M
LEHRACH, H
[J]. TRENDS IN GENETICS, 1989, 5 (02) : 41 - 41
[9] ALTERING THE GENOME BY HOMOLOGOUS RECOMBINATION
CAPECCHI, MR
[J]. SCIENCE, 1989, 244 (4910) : 1288 - 1292
[10] CHAMPOUX JJ, 1988, GENETIC RECOMBINATIO, P655

← 1 2 3 4 5 6 →