MODULATION OF NA-H ANTIPORTER ACTIVITY IN HUMAN LYMPHOBLASTS BY ALTERED MEMBRANE CHOLESTEROL

被引：17

作者：

DEFIGUEIREDO, CEP ^{[1
]}

NG, LL ^{[1
]}

DAVIS, JE ^{[1
]}

LUCIOCAZANA, FJ ^{[1
]}

ELLORY, JC ^{[1
]}

HENDRY, BM ^{[1
]}

机构：

[1] UNIV OXFORD, NUFFIELD DEPT CLIN MED, OXFORD OX1 3PT, ENGLAND

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 261卷 / 06期

关键词：

FLUORESCENCE; INTRACELLULAR PH; MEMBRANE LIPID;

D O I：

10.1152/ajpcell.1991.261.6.C1138

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The effects of changes in membrane cholesterol on Na-H antiporter activity in cultured human lymphoblasts are described. Lymphoblast cholesterol alteration was achieved with liposomes of phosphatidylcholine (cholesterol depletion) or phosphatidylcholine plus cholesterol (cholesterol enrichment). Lymphoblast intracellular pH (pH(i)) was examined by fluorimetry using cells loaded with the pH-sensitive dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein, and the Na-dependent proton efflux rate at a pH(i) of 6.0 was taken as the maximum velocity of the Na-H antiporter. Lymphoblast membrane cholesterol depletion activated the Na-H antiporter, and enrichment of membrane cholesterol caused inhibition of the antiporter activity. This study demonstrates that in situ modification of membrane cholesterol can modulate the activity of the Na-H antiporter.

引用

页码：C1138 / C1142

页数：5

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