CELL-DIVISION ARREST INDUCED BY PHORBOL ESTER IN CHO CELLS OVEREXPRESSING PROTEIN KINASE-C-DELTA SUBSPECIES

被引：321

作者：

WATANABE, T

ONO, Y

TANIYAMA, Y

HAZAMA, K

IGARASHI, K

OGITA, K

KIKKAWA, U

NISHIZUKA, Y

机构：

[1] KOBE UNIV,BIOSIGNAL RES CTR,KOBE 657,JAPAN

[2] KOBE UNIV,SCH MED,DEPT BIOCHEM,KOBE 650,JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 21期

关键词：

D O I：

10.1073/pnas.89.21.10159

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Several lines of CHO cells stably overexpressing protein kinase C (PKC) subspecies to various extents were established by the DNA-mediated transfer. Upon treatment with phorbol 12-myristate 13-acetate, the growth of the cells expressing the PKC-delta subspecies was markedly inhibited, whereas cell lines expressing PKC-alpha, PKC-betaII, and PKC-zeta subspecies were not significantly affected. Flow cytometric analysis indicated that all cell lines overexpressing PKC-delta subspecies accumulated in G2/M phase in response to phorbol 12-myristate 13-acetate. In these arrested cells, dikaryons were predominant, implying that phorbol ester-induced inhibition of cell division is specific to telophase. These results suggest PKC-delta subspecies may play a role in the normal cell cycle progression.

引用

页码：10159 / 10163

页数：5

共 38 条

[1] ISOLATION AND CHARACTERIZATION OF PKC-L, A NEW MEMBER OF THE PROTEIN-KINASE C-RELATED GENE FAMILY SPECIFICALLY EXPRESSED IN LUNG, SKIN, AND HEART [J].