PROTEIN-F, A FIBRONECTIN-BINDING PROTEIN, IS AN ADHESIN OF THE GROUP-A STREPTOCOCCUS STREPTOCOCCUS-PYOGENES

被引:260
作者
HANSKI, E [1 ]
CAPARON, M [1 ]
机构
[1] WASHINGTON UNIV, SCH MED, DEPT MOLEC MICROBIOL, ST LOUIS, MO 63110 USA
关键词
FIBRONECTIN RECEPTOR; MICROBIAL ADHERENCE; VIRULENCE;
D O I
10.1073/pnas.89.13.6172
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Binding to fibronectin has been suggested to play an important role in adherence of the group A streptococcus Streptococcus pyogenes to host epithelial cells; however, the identity of the streptococcal fibronectin, receptor has been elusive. Here we demonstrate that the fibronectin-binding property of S. pyogenes is mediated by protein F, a bacterial surface protein that binds fibronectin at high affinity. The gene encoding protein F (prtF) produced a functional fibronectin-binding protein in Escherichia coli. Insertional mutagenesis of the cloned gene generated a mutation that resulted in the loss of fibronectin-binding activity. When this mutation was introduced into the S. pyogenes chromosome by homologous recombination with the wild-type allele, the resulting strains no longer produced protein F and lost their ability to bind fibronectin. The mutation could be complemented by prtF introduced on a plasmid. Mutants lacking protein F had a much lower capacity to adhere to respiratory epithelial cells. These results demonstrate that protein F is an important adhesin of S. pyogenes.
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页码:6172 / 6176
页数:5
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