IDENTITY OF THE MAJOR CYSTEINE PROTEINASE (CRUZIPAIN) FROM TRYPANOSOMA-CRUZI AND AN ANTIGEN (AG163B6) ISOLATED WITH A MONOCLONAL-ANTIBODY

被引：20

作者：

MALCHIODI, EL ^{[1
]}

CHIARAMONTE, MG ^{[1
]}

MARTINEZ, JA ^{[1
]}

ZWIRNER, NW ^{[1
]}

MARGNI, RA ^{[1
]}

CAZZULO, JJ ^{[1
]}

机构：

[1] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,CONICET,FDN CAMPOMAR,BUENOS AIRES,ARGENTINA

来源：

IMMUNOLOGY LETTERS | 1993年 / 35卷 / 01期

关键词：

CRUZIPAIN; AG163B6;

D O I：

10.1016/0165-2478(93)90148-U

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-PAGE, Western blotting, isoelectric focusing, two-dimensional electrophoresis (IEF and SDS-PAGE), Ouchterlony's double diffusion, and enzyme activity in SDS-PAGE gels containing 0.1% gelatin suggests that they are identical.

引用

页码：59 / 62

页数：4

共 18 条

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