A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR THE DETERMINATION OF ITRACONAZOLE CONCENTRATION USING SOLID-PHASE EXTRACTION AND SMALL SAMPLE VOLUME

被引:18
作者
RIFAI, N
SAKAMOTO, M
PLATT, O
BRUGNARA, C
机构
[1] CHILDRENS HOSP,DEPT PATHOL,BOSTON,MA 02115
[2] CHILDRENS HOSP,DEPT MED,BOSTON,MA 02115
[3] HARVARD UNIV,SCH MED,BOSTON,MA
关键词
ITRACONAZOLE; PLASMA CONCENTRATION; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ASSAY; SOLID-PHASE EXTRACTION;
D O I
10.1097/00007691-199510000-00014
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Itraconazole has been shown to be highly effective against a broad spectrum of fungi, particularly aspergillus. In order to achieve the desired pharmacological effect, the itraconazole plasma concentration should be >250 ng/ml. We developed a high-performance liquid chromatography assay for the rapid determination of plasma itraconazole concentration using solid-phase extraction and small sample volume. The assay possessed linearity up to 5,000 ng/ml, sensitivity of 50 ng/ml, average recovery of 100.8%, and run-to-run precision (n = 10) for concentrations of 100, 200, and 500 ng/ml of 12, 6.6, and 5.8%, respectively. Furthermore, the assay proved to be free of interference from 48 commonly prescribed and some over-the-counter medications. We conclude that the method described here is ideally suited for the therapeutic monitoring of itraconazole.
引用
收藏
页码:522 / 525
页数:4
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