YEAST KRE1 IS A CELL-SURFACE O-GLYCOPROTEIN

被引：29

作者：

ROEMER, T ^{[1
]}

BUSSEY, H ^{[1
]}

机构：

[1] MCGILL UNIV, DEPT BIOL, MONTREAL, PQ H3A 1B1, CANADA

来源：

MOLECULAR AND GENERAL GENETICS | 1995年 / 249卷 / 02期

关键词：

YEAST; CELL WALL; BETA-GLUCAN SYNTHESIS; KRELP; O-GLYCOPROTEIN;

D O I：

10.1007/BF00290368

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Saccharomyces cerevisiae KRE1 gene encodes a secretory protein required for the production of the cell wall polymer (1 --> 6)-beta-glucan. Here we report further characterization of the KRE1 gene product, Kre1p. A functional, epitope-tagged Kre1p is shown to be highly modified in a SEC53-dependent manner. Kre1p is O-glycosylated, but the basis for the majority of its post-translational modification is unknown. Fractionation of Kre1p reveals a cell wall-associated form and a less abundant membrane-associated species. Indirect immunoflurorescence demonstrates that Kre1p localizes to the cell surface, where it becomes concentrated at the surface of mother cells. Such a localization of Kre1p seems to parallel the CAL1/CSD2-dependent cell wall deposition of chitin found in S. cerevisiae, and is consistent with evidence from Schizophyllum commune that (1 --> 6)-beta-glucan accumulates during maturation of the subapical region of the wall distal to the hyphal tip.

引用

页码：209 / 216

页数：8

共 39 条

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