RECEPTOR FUNCTIONS AND LIGAND-DEPENDENT TRANSFORMING POTENTIAL OF A CHIMERIC KIT PROTOONCOGENE

被引:36
作者
LEV, S [1 ]
YARDEN, Y [1 ]
GIVOL, D [1 ]
机构
[1] WEIZMANN INST SCI,DEPT CHEM IMMUNOL,IL-76100 REHOVOT,ISRAEL
关键词
D O I
10.1128/MCB.10.11.6064
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The c-kit proto-oncogene, the cellular homolog of the transforming gene of a feline retrovirus, encodes a transmembrane tyrosine kinase homologous to receptors for growth factors. To study the cellular function of c-kit, we constructed a chimeric molecule composed of the extracellular portion of the receptor for epidermal growth factor (EGF) and the transmembrane and cytoplasmic domains of p145(kit). The hybrid molecule was properly expressed in murine fibroblasts and displayed specific binding of EGF (K(d), 3 x 10-8 M). Activation of the chimeric receptor by EGF stimulated the tyrosine kinase activity of kit and led to the generation of a potent mitogenic signal. Moreover, cells expressing the chimeric receptor acquired a transformed phenotype once they were stimulated with the heterologous ligand.
引用
收藏
页码:6064 / 6068
页数:5
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