ELECTROPERMEABILIZATION OF HUMAN CULTURED-CELLS GROWN IN MONOLAYERS - INCORPORATION OF MONOCLONAL-ANTIBODIES

被引：16

作者：

KWEE, S ^{[1
]}

NIELSEN, HV ^{[1
]}

CELIS, JE ^{[1
]}

机构：

[1] AARHUS UNIV,BIOREGULAT RES CTR,DK-8000 AARHUS C,DENMARK

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1990年 / 23卷 / 01期

关键词：

D O I：

10.1016/0302-4598(90)80005-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cultured cells grown in monolayers were permeabilized by applying low-voltage electric pulses. The permeabilized cells were able to incorporate extracellular nucleotides into their DNA, as well as various monoclonal antibodies as determined by indirect immunofluorescence. Complete reversibility and long term viability were retained. Both applied voltage and pulse duration were important parameters for this method and the results were different for different cell lines. © 1990.

引用

页码：65 / 80

页数：16

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