THE POLYMERASE CHAIN-REACTION FOR MYCOPLASMA-PULMONIS

被引：18

作者：

HARASAWA, R ^{[1
]}

KOSHIMIZU, K ^{[1
]}

UEMORI, T ^{[1
]}

TAKEDA, O ^{[1
]}

ASADA, K ^{[1
]}

KATO, I ^{[1
]}

机构：

[1] TAKARA SHUZO CO LTD,BIOTECHNOL RES LAB,OTSU,SHIGA 52021,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 34卷 / 04期

关键词：

D O I：

10.1111/j.1348-0421.1990.tb01020.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In vitro DNA amplification by polymerase chain reaction was examined to detect Mycoplasma pulmonis. A pair of synthetic oligonucleotide primers was constructed, and used to amplify a unique sequence of M. pulmonis DNA. Amplified products were detected by agarose gel electrophoresis and verified by blot hybridization with a synthetic oligonucleotide probe. This system detected cellular DNA of M. pulmonis but not M. arthritidis or M. neurolyticum, and thus appears to be useful for M. pulmonis diagnosis. © 1990, Center For Academic Publications Japan. All rights reserved.

引用

页码：393 / 395

页数：3

共 8 条

[1]

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