C-MYC EXPRESSION IS DOWN-REGULATED BY CELL-CELL AND CELL-EXTRACELLULAR MATRIX CONTACTS IN NORMAL HEPATOCYTES, BUT NOT IN HEPATOMA-CELLS

被引：25

作者：

SHIMBARA, N ^{[1
]}

TAKASHINA, M ^{[1
]}

SATO, C ^{[1
]}

IIZUKA, M ^{[1
]}

KOBAYASHI, S ^{[1
]}

TANAKA, K ^{[1
]}

ICHIHARA, A ^{[1
]}

机构：

[1] UNIV TOKUSHIMA,INST ENZYME RES,TOKUSHIMA 770,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90664-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Primary culture of adult rat hepatocytes resulted in marked increase of c-myc expression within a few hours. The high level of c-myc mRNA was maintained throughout culture on collagen-coated dishes, but decreased greatly with time during culture on collagen-gel or matrigel. Expression of c-myc was also down-regulated at high cell density. The decrease in its expression appeared closely related to inhibitions of DNA synthesis and cell spreading. In contrast, hepatoma H4TG cells showed a high level of c-myc expression which was not affected by culture on any extracellular matrices examined or by the cell density. These results suggest that upregulation of expression of the c-myc gene is linked to G0 to G1 transition during cell cycle progression, which in normal hepatocytes is strictly regulated by cell-cell and cell-extracellular matrix interactions, but that this control mechanism is defective in malignant hepatic tumor cells. © 1992.

引用

页码：825 / 831

页数：7

共 16 条

[1] CELL CELL AND CELL MATRIX INTERACTIONS DIFFERENTIALLY REGULATE THE EXPRESSION OF HEPATIC AND CYTOSKELETAL GENES IN PRIMARY CULTURES OF RAT HEPATOCYTES [J].