IDENTIFICATION OF DRUG GLUCURONIDES IN HUMAN URINE BY RP-HPLC AFTER DERIVATIZATION

被引：6

作者：

KOHL, C ^{[1
]}

OELSCHLAGER, H ^{[1
]}

ROTHLEY, D ^{[1
]}

机构：

[1] JOHANN WOLFGANG GOETHE UNIV,INST PHARMAZEUT CHEM,W-6000 FRANKFURT 1,GERMANY

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1994年 / 12卷 / 02期

关键词：

DERIVATIVES OF DRUG GLUCURONIDES; RP-HPLC; CLOFIBRATE; PHENAZONE; DISULFIRAM; SULFAMETHOXAZOLE;

D O I：

10.1016/0731-7085(94)90036-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the identification of four types of drug glucuronides in human urine is presented. The approach involves solid-phase extraction (C18 columns) from acidified human urine and subsequent methylation and acetylation of the extracted drug glucuronides to triacetyl methyl derivatives. These derivatives were identified by RP-HPLC by comparison with synthesized authentic reference compounds. The scope of the method was demonstrated by identification of glucuronides formed by metabolism of clofibrate, phenazone, disulfiram and sulfamethoxazole in urine samples of two male volunteers.

引用

页码：249 / 254

页数：6

共 30 条

[1]

AGAKI M, 1966, CHEM PHARM BULL, V14, P1

[2]

AHMAD B, 1988, INDIAN J PHARM SCI, P291

[3] ENZYMIC-SYNTHESIS OF 2 GLUCURONIDES OF THE HYDROXYISOXAZOLE GABA-AGONIST, THIP, AND THE INVIVO GLUCURONIDATION OF THIP IN RAT [J].

ANDERSEN, JV ;

DALGAARD, L ;

HANSEN, SH .

XENOBIOTICA, 1989, 19 (12) :1399-1406

[4]

ANGYAL SJ, 1972, CARBOHYDRATES A, V1, P200

[5] THE SYNTHESIS OF ARYL-D-GLUCOPYRANOSIDURONIC ACIDS [J].

BOLLENBACK, GN ;

LONG, JW ;

BENJAMIN, DG ;

LINDQUIST, JA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1955, 77 (12) :3310-3315

[6]

FEIL VJ, 1986, ACS SYM SER, V299, P177

[7]

FENSELAU C, 1980, DRUG METAB DISPOS, V8, P274

[8]

GOENECHEA S, 1978, ARZNEIMITTEL-FORSCH, V28-2, P1070

[9] STUDIES ON THE CLEAVAGE OF GLUCURONIDES OF SOME MAJOR METABOLITES OF METHAQUALONE WITH HYDROCHLORIC-ACID [J].

GOENECHEA, S ;

WESSLING, W ;

GOEBEL, KJ .

FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE, 1983, 315 (04) :359-360

[10]

HALL LD, 1980, CARBOHYDRATES CHEM B, V1, P1299

← 1 2 3 →