THE USE OF ON-LINE DIALYSIS WITH HPLC FOR THE AUTOMATED PREPARATION AND ANALYSIS OF COMPLEX SAMPLES.

Dialysis has recently become a very efficient pre-chromatographic treatment technique for the determination of small and medium molecules in large series of biological, environmental and food samples when the elimination of macromolecules and solid particles is required.This paper will discuss how the classical limitations of dialysis have been overcome to achieve a recovery of 60 to 90% of the starting analytes within 3 to 7 minutes. Analytical performances are discussed within the context of automated routine analyses using HDOC.