SIMPLE AND EFFICIENT PURIFICATION OF C-REACTIVE PROTEIN FROM CANINE SERUM

被引：6

作者：

ONISHI, T

SHIMIZU, T

KAJIKAWA, T

机构：

[1] UNIV OSAKA PREFECTURE,COLL AGR,DEPT VET INTERNAL MED,SAKAI,OSAKA 593,JAPAN

[2] UNIV OSAKA PREFECTURE,COLL AGR,DEPT VET MICROBIOL,SAKAI,OSAKA 593,JAPAN

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1994年 / 56卷 / 02期

关键词：

AFFINITY CHROMATOGRAPHY; CANINE SERUM; C-REACTIVE PROTEIN;

D O I：

10.1292/jvms.56.417

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Simple purification method for canine serum, C-reactive protein (CRP) was established. By combining an affinity chromatography on Sepharose 4B coupled with o-phosphorylethanolamine and a gel filtration on a column of Sephacryl S-300, about 9 mg of purified CRP from 50 ml of canine serum was obtained. The method is simple and efficient to purify canine CRP.

引用

页码：417 / 419

页数：3

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[2]

CASPI D, 1984, IMMUNOLOGY, V53, P307

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