CONTINUOUS ELECTROENZYMATIC SYNTHESIS EMPLOYING THE ELECTROCHEMICAL ENZYME MEMBRANE REACTOR

被引：28

作者：

BRIELBECK, B

FREDE, M

STECKHAN, E

机构：

[1] Institut für Organische Chemie und Biochemie der, Universität Bonn, D-53121, Bonn

来源：

BIOCATALYSIS | 1994年 / 10卷 / 1-4期

关键词：

ELECTROENZYMATIC SYNTHESIS; ELECTROCHEMICAL ENZYME MEMBRANE REACTOR; CONTINUOUS ENZYME REACTIVATION; CONTINUOUS SYNTHESIS;

D O I：

10.3109/10242429409065217

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have applied flavocytochromes in a continuous synthesis of organic compounds. The removal of the product was accomplished with a membrane reactor without interuption of the synthetic reaction. The prosthetic groups were electrochemically reactivated in a flow cell with water soluble, polymer modified ferrocene derivatives as mediators. Turnover numbers of up to 400,000 were achieved without deactivation of either enzyme or mediator.

引用

收藏

页码：49 / 64

页数：16

相关论文

共 15 条

[1] FERROCENE-MEDIATED ENZYME ELECTRODE FOR AMPEROMETRIC DETERMINATION OF GLUCOSE [J].

CASS, AEG ;

DAVIS, G ;

FRANCIS, GD ;

HILL, HAO ;

ASTON, WJ ;

HIGGINS, IJ ;

PLOTKIN, EV ;

SCOTT, LDL ;

TURNER, APF .

ANALYTICAL CHEMISTRY, 1984, 56 (04) :667-671

[2]

DRUECKHAMMER DG, 1991, SYNTHESIS-STUTTGART, P499

[3] ARTIFICIAL METABOLISMS FOR THE ASYMMETRIC ONE-POT SYNTHESIS OF BRANCHED-CHAIN SACCHARIDES [J].

FESSNER, WD ;

WALTER, C .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1992, 31 (05) :614-616

[4]

FESSNER WD, 1992, ANGEW CHEM, V104, P63

[5] CONTINUOUS ELECTROCHEMICAL ACTIVATION OF FLAVOENZYMES USING POLYETHYLENEGLYCOL-BOUND FERROCENES AS MEDIATORS - A MODEL FOR THE APPLICATION OF OXIDOREDUCTASES AS OXIDATION CATALYSTS IN ORGANIC-SYNTHESIS [J].

FREDE, M ;

STECKHAN, E .

TETRAHEDRON LETTERS, 1991, 32 (38) :5063-5066

[6]

JONES JB, 1985, ORG SYNTH, V63, P10

[7] CONTINUOUS-PROCESSES WITH SOLUBLE ENZYMES [J].

KRAGL, U ;

VASICRACKI, D ;

WANDREY, C .

CHEMIE INGENIEUR TECHNIK, 1992, 64 (06) :499-509

[8] PARA-CRESOL METHYLHYDROXYLASE - ASSAY AND GENERAL-PROPERTIES [J].

MCINTIRE, W ;

HOPPER, DJ ;

SINGER, TP .

BIOCHEMICAL JOURNAL, 1985, 228 (02) :325-335

[9] STEREOCHEMICAL ASPECTS OF THE OXIDATION OF 4-ETHYLPHENOL BY THE BACTERIAL ENZYME 4-ETHYLPHENOL METHYLENEHYDROXYLASE [J].

REEVE, CD ;

CARVER, MA ;

HOPPER, DJ .

BIOCHEMICAL JOURNAL, 1990, 269 (03) :815-819

[10] THE PURIFICATION AND CHARACTERIZATION OF 4-ETHYLPHENOL METHYLENE-HYDROXYLASE, A FLAVOCYTOCHROME FROM PSEUDOMONAS PUTIDA JD1 [J].

REEVE, CD ;

CARVER, MA ;

HOPPER, DJ .

BIOCHEMICAL JOURNAL, 1989, 263 (02) :431-437