DEVELOPMENT OF A DETECTION SYSTEM FOR VIRUSES OF WOODY-PLANTS BASED ON PCR ANALYSIS OF IMMOBILIZED VIRIONS

被引：113

作者：

ROWHANI, A ^{[1
]}

MANINGAS, MA ^{[1
]}

LILE, LS ^{[1
]}

DAUBERT, SD ^{[1
]}

GOLINO, DA ^{[1
]}

机构：

[1] USDA ARS,NATL GERMPLASM REPOSITORY,DAVIS,CA 95616

来源：

PHYTOPATHOLOGY | 1995年 / 85卷 / 03期

关键词：

ANTIGEN CAPTURE PCR; APPLE MOSAIC VIRUS; CHERRY LEAFROLL VIRUS; CITRUS TRISTEZA VIRUS; GRAPEVINE FANLEAF VIRUS; PRUNE DWARF VIRUS; PRUNUS NECROTIC RINGSPOT VIRUS;

D O I：

10.1094/Phyto-85-347

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The genomes of several viruses infecting woody host plants were found to serve as templates for the reverse transcriptase-polymerase chain reaction (RT-PCR) while still contained within their capsids as virions in solution. A detection system was developed in which the virions could be assayed by RT-PCR after recovery from solution by immobilization directly onto polypropylene or polystyrene surfaces. Components of crude plant extracts that could otherwise inhibit the RT-PCR analysis were washed away, and the immobilized virions could then be detected by the PCR reaction. Quantitative RT-PCR assays of virions bound nonspecifically to plastic were compared to those in which the viruses were bound by specific antiserum-directed immunocapture. Binding to plastic was inhibited to a greater extent by extracts of some of the host plants than was immunocapture.

引用

页码：347 / 352

页数：6

共 30 条

[1] THE COMPLETE NUCLEOTIDE-SEQUENCE OF PRUNE DWARF ILARVIRUS RNA-3 - IMPLICATIONS FOR COAT PROTEIN-ACTIVATION OF GENOME REPLICATION IN ILARVIRUSES [J].