TRANSFORMATION STUDIES IN HORDEUM-VULGARE USING A HIGHLY REGENERABLE MICROSPORE SYSTEM

被引：18

作者：

HARWOOD, WA

BEAN, SJ

CHEN, DF

MULLINEAUX, PM

SNAPE, JW

机构：

[1] John Innes Centre, Norwich, NR4 7UH, Colney

来源：

EUPHYTICA | 1995年 / 85卷 / 1-3期

关键词：

HORDEUM VULGARE; ISOLATED MICROSPORES; PARTICLE BOMBARDMENT; TRANSFORMATION;

D O I：

10.1007/BF00023938

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A highly regenerable, isolated microspore system for barley, Hordeum vulgare L. cv. Igri, has been developed which is amenable to transformation studies using particle bombardment. The system allows DNA to be delivered to microspores at the single cell stage and both transient and stable transformation events have been demonstrated. The potential advantages of using isolated microspores as the target tissue in routine transformation systems are discussed.

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页码：113 / 118

页数：6

相关论文

共 24 条

[1] FERTILE TRANSGENIC WHEAT FROM MICROPROJECTILE BOMBARDMENT OF SCUTELLAR TISSUE [J].

BECKER, D ;

BRETTSCHNEIDER, R ;

LORZ, H .

PLANT JOURNAL, 1994, 5 (02) :299-307

[2] IDENTIFICATION OF EMBRYOGENIC MICROSPORES OF BARLEY (HORDEUM-VULGARE L) BY INDIVIDUAL SELECTION AND CULTURE AND THEIR POTENTIAL FOR TRANSFORMATION BY MICROINJECTION [J].

BOLIK, M ;

KOOP, HU .

PROTOPLASMA, 1991, 162 (01) :61-68

[3] PRODUCTION OF TRANSGENIC RICE (ORYZA-SATIVA L) PLANTS FROM AGRONOMICALLY IMPORTANT INDICA AND JAPONICA VARIETIES VIA ELECTRIC-DISCHARGE PARTICLE-ACCELERATION OF EXOGENOUS DNA INTO IMMATURE ZYGOTIC EMBRYOS [J].

CHRISTOU, P ;

FORD, TL ;

KOFRON, M .

BIO-TECHNOLOGY, 1991, 9 (10) :957-962

[4] AGROBACTERIUM - AND MICROPROJECTILE - MEDIATED VIRAL-DNA DELIVERY INTO BARLEY MICROSPORE-DERIVED CULTURES [J].

CREISSEN, G ;

SMITH, C ;

FRANCIS, R ;

REYNOLDS, H ;

MULLINEAUX, P .

PLANT CELL REPORTS, 1990, 8 (11) :680-683

[5]

FOROUGHIWEHR B, 1976, Z PFLANZENZUCHT, V77, P198

[6] INHERITANCE AND EXPRESSION OF CHIMERIC GENES IN THE PROGENY OF TRANSGENIC MAIZE PLANTS [J].

FROMM, ME ;

MORRISH, F ;

ARMSTRONG, C ;

WILLIAMS, R ;

THOMAS, J ;

KLEIN, TM .

BIO-TECHNOLOGY, 1990, 8 (09) :833-839

[7]

GORDONKAMM WJ, 1990, PLANT CELL, V2, P603, DOI 10.1105/tpc.2.7.603

[8] MICROSPORE CULTURE OF HORDEUM-VULGARE L - THE INFLUENCE OF DENSITY AND OSMOLALITY [J].

HOEKSTRA, S ;

VANZIJDERVELD, MH ;

HEIDEKAMP, F ;

VANDERMARK, F .

PLANT CELL REPORTS, 1993, 12 (12) :661-665

[9] ANTHER AND MICROSPORE CULTURE OF HORDEUM-VULGARE L CV IGRI [J].

HOEKSTRA, S ;

VANZIJDERVELD, MH ;

LOUWERSE, JD ;

HEIDEKAMP, F ;

VANDERMARK, F .

PLANT SCIENCE, 1992, 86 (01) :89-96

[10]

Hunter C. P., 1987, European Patent Application, VAPPL, Patent No. [0245898A2, 0245898]