C-SRC GENE-PRODUCT IN DEVELOPING RAT-BRAIN IS ENRICHED IN NERVE GROWTH CONE MEMBRANES

被引：212

作者：

MANESS, PF ^{[1
]}

AUBRY, M ^{[1
]}

SHORES, CG ^{[1
]}

FRAME, L ^{[1
]}

PFENNINGER, KH ^{[1
]}

机构：

[1] UNIV COLORADO, SCH MED, DEPT CELLULAR & STRUCT BIOL, DENVER, CO 80262 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1988年 / 85卷 / 14期

关键词：

D O I：

10.1073/pnas.85.14.5001

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Differentiating rat neurons express high levels of the protooncogene product pp60c-src, a 60-kDa tyrosine kinase of unknown function encoded by c-src. pp60c-src was found to be concentrated at least 9-fold in membranes from a subcellular fraction of nerve growth cones, the motile tips of outgrowing neuronal processes. Indirect immunofluorescence staining of cultured chick retinal explants showed pp60c-src in neuronal growth cones and processes, with the antigen particularly concentrated in growth cones of long neurites. pp60c-src in growth cone membranes was an active tyrosine-specific protein kinase with elevated tyrosine-specific protein kinase activity and reduced electrophoretic mobility characteristic of the form of pp60c-src in central nervous system neurons. pp60c-src was present at lower levels in subcellular fractions from mature rat brain but synaptosomal membranes were not enriched. Preferential localization of an active form of pp60C-srC in nerve growth cone membranes and persistence of pp60c-src in mature neurons suggest that this tyrosine kinase is important in growth cone-mediated neurite extension and synaptic plasticity.

引用

页码：5001 / 5005

页数：5

共 42 条

[1] DIFFERENTIATION OF PC12 PHEOCHROMOCYTOMA CELLS INDUCED BY V-SRC ONCOGENE [J].

ALEMA, S ;

CASALBORE, P ;

AGOSTINI, E ;

TATO, F .

NATURE, 1985, 316 (6028) :557-559

[2]

AMES BN, 1960, J BIOL CHEM, V235, P769

[3] INCREASED PP60C-SRC TYROSYL KINASE-ACTIVITY IN HUMAN NEUROBLASTOMAS IS ASSOCIATED WITH AMINO-TERMINAL TYROSINE PHOSPHORYLATION OF THE SRC GENE-PRODUCT [J].

BOLEN, JB ;

ROSEN, N ;

ISRAEL, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (21) :7275-7279

[4] NEURONS EXPRESS HIGH-LEVELS OF A STRUCTURALLY MODIFIED, ACTIVATED FORM OF PP60C-SRC [J].

BRUGGE, JS ;

COTTON, PC ;

QUERAL, AE ;

BARRETT, JN ;

NONNER, D ;

KEANE, RW .

NATURE, 1985, 316 (6028) :554-557

[5] MYRISTIC ACID IS ATTACHED TO THE TRANSFORMING PROTEIN OF ROUS-SARCOMA VIRUS DURING OR IMMEDIATELY AFTER SYNTHESIS AND IS PRESENT IN BOTH SOLUBLE AND MEMBRANE-BOUND FORMS OF THE PROTEIN [J].

BUSS, JE ;

KAMPS, MP ;

SEFTON, BM .

MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (12) :2697-2704

[6] ALTERATIONS IN PP60C-SRC ACCOMPANY DIFFERENTIATION OF NEURONS FROM RAT EMBRYO STRIATUM [J].

CARTWRIGHT, CA ;

SIMANTOV, R ;

KAPLAN, PL ;

HUNTER, T ;

ECKHART, W .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (05) :1830-1840

[7] STRUCTURE OF POSTSYNAPTIC DENSITIES ISOLATED FROM DOG CEREBRAL-CORTEX .1. OVERALL MORPHOLOGY AND PROTEIN-COMPOSITION [J].

COHEN, RS ;

BLOMBERG, F ;

BERZINS, K ;

SIEKEVITZ, P .

JOURNAL OF CELL BIOLOGY, 1977, 74 (01) :181-203

[8] PROTEIN-KINASE ACTIVITY ASSOCIATED WITH AVIAN-SARCOMA VIRUS SRC GENE PRODUCT [J].

COLLETT, MS ;

ERIKSON, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (04) :2021-2024

[9] CHARACTERIZATION OF A NORMAL AVIAN CELL PROTEIN RELATED TO THE AVIAN-SARCOMA VIRUS TRANSFORMING GENE PRODUCT [J].

COLLETT, MS ;

BRUGGE, JS ;

ERIKSON, RL .

CELL, 1978, 15 (04) :1363-1369

[10] NEURAL TISSUES EXPRESS HIGH-LEVELS OF THE CELLULAR SRC GENE-PRODUCT PP60C-SRC [J].

COTTON, PC ;

BRUGGE, JS .

MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (06) :1157-1162

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