A PHYSICAL MAP AROUND THE RETINOBLASTOMA GENE

被引：18

作者：

FORD, GM ^{[1
]}

GALLIE, BL ^{[1
]}

PHILLIPS, RA ^{[1
]}

BECKER, AJ ^{[1
]}

机构：

[1] HOSP SICK CHILDREN,RES INST,TORONTO M5G 1X8,ONTARIO,CANADA

来源：

GENOMICS | 1990年 / 6卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0888-7543(90)90568-F

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene predisposing to retinoblastoma, RB1, has been mapped to 13q14 and a cDNA clone has been isolated. Alterations of this chromosomal region are found not only in retinoblastoma, but in other tumor types including bone and soft tissue sarcomas, gastric tumors, small cell lung cancer, hematologic malignancies, rhabdomyosarcoma, and breast cancer. Genetic alterations implicating RB1 in some of these cancers have been observed. A long-range, overlapping restriction map around RB1 has been derived to provide a basis for study of rearrangements in tumors. Putative CpG islands closely linked to RB1 were identified, the effect of methylation was investigated, and RB1 transcriptional direction was determined. Using data in the literature, the map was oriented with respect to the centromere and it was determined that the distance between esterase D, a nearby gene, and RB1 was greater than 200 kb. © 1990.

引用

页码：284 / 292

页数：9

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