CHEMICALLY BONDED PHASES FOR THE REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF BASIC SUBSTANCES

被引：118

作者：

BUSZEWSKI, B

SCHMID, J

ALBERT, K

BAYER, E

机构：

[1] UNIV TUBINGEN,INST ORGAN CHEM,MORGENSTELLE 18,W-7400 TUBINGEN 1,GERMANY

[2] MARIE CURIE SKLODOWSKA UNIV,FAC CHEM,DEPT CHEM PHYS,PL-20031 LUBLIN,POLAND

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 552卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)95957-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A chemically bonded phase with a peptide group (PB) for reversed-phase high-performance liquid chromatography (HPLC) is described. This packing was prepared by a two-stage modification of the surface of silica gel with mono- and trifunctional 3-aminopropylsilane and then with an appropriate derivative of a fatty acid. Packings prepared in this way were compared with standard C18 materials used in HPLC. Surface characteristics of the packings before and after chemical modification were determined by different physico-chemical methods, e.g., porosimetry, elemental analysis, C-13 and Si-29 cross-polarization magic angle spinning NMR and HPLC. Chromatographic properties of these packings were evaluated by comparison between log k' of one phase and log k' of a second phase for substances with different chemical natures. The PB packing was found to be especially useful for the separation of basic substances.

引用

页码：415 / 427

页数：13

共 30 条

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