A PROCEDURE FOR THE EXTRACTION OF CHLOROPLAST DNA FROM BROAD-LEAVED TREE SPECIES

被引：11

作者：

MICHAUD, H ^{[1
]}

LUMARET, R ^{[1
]}

RIPOLL, JP ^{[1
]}

TOUMI, L ^{[1
]}

机构：

[1] CTR ECOL FONCT & EVOLUT LOUIS EMBERGER,DEPT POPULAT BIOL,CNRS,F-34033 MONTPELLIER,FRANCE

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1995年 / 13卷 / 02期

关键词：

CHLOROPLAST DNA; BROAD-LEAVED TREE SPECIES; RFLP;

D O I：

10.1007/BF02668784

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the extraction of ctDNA from isolated chloroplast was developed. This method is simple and adapted particularly to broad-leaved trees, including sclerophyllous species with high phenolic and polysaccharide contents. This method includes two major steps: first, chloroplasts are isolated in non-aqueous solutions to avoid oxidation and phenolic problems; second, ctDNA is extracted from the chloroplasts using aqueous solutions and specific methods to provide highly purified ctDNA.

引用

页码：131 / 137

页数：7

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