THE INFLUENCE OF HUMAN FOLLICULAR-FLUID ON THE ACROSOME REACTION, FERTILIZING-CAPACITY AND PROTEINASE ACTIVITY OF HUMAN SPERMATOZOA

被引：38

作者：

SIEGEL, MS

PAULSON, RJ

GRACZYKOWSKI, JW

机构：

[1] University of Southern California School of Medicine, Department of Obstetrics and Gynecology, Los Angeles

来源：

HUMAN REPRODUCTION | 1990年 / 5卷 / 08期

关键词：

FOLLICULAR FLUID; ACROSOME; HAMSTER PENETRATION TEST; SPERM PROTEINASES; FERTILIZATION;

D O I：

10.1093/oxfordjournals.humrep.a137231

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The present study was designed to assess physiological and enzymatic changes in human spermatozoa following incubation in human follicular fluid (HFF). Initially, it was determined that infertility patients (n = 29) scored dramatically higher on the hamster egg penetration test (HEPT) when spermatozoa were incubated with HFF (22.9 +/- 4.4%) rather than the standard swim-up alone (4.6 +/- 1.1%). To further evaluate this effect, in experiment I, spermatozoa were obtained from proven fertile individuals and evaluated following exposure to three different HFF samples as well as control treatments (medium, cul de sac fluid and heparin). There were no significant differences in HEPT scores following sperm incubation in the three different follicular fluids although incubation in all the fluids significantly (P < 0.01) enhanced sperm penetration (%PEN) when compared to the standard swim-up and other control treatments. The absence of an effect of cul de sac fluid on spermatozoa indicates that not all body fluids contain factors which stimulate sperm fertilizing capacity. The effect of HFF was demonstrated in a infertile patient population as well as in donors of proven fertility. In experiment II, the effect of HFF on the acrosome reaction (%AR), sperm fertilizing capacity and changes in sperm proteolytic enzymes were determined. There was no significant difference in the %AR between freshly ejaculated (7.9 +/- 3.1) and medium incubated (9.4 +/- 1.6) spermatozoa; however, in both of these treatments the %AR was less (P < 0.01) than for spermatozoa treated with HFF (45.6 +/- 4.7). The %PEN following incubation of spermatozoa in HFF (52.2 +/- 6.8) was greatly increased (P +/- 0.01) compared to the standard swim-up (19 +/- 3.9). There was also a decrease (P +/- 0.01) in trypsin-like enzyme activity in spermatozoa following incubation in HFF (53.6 +/- 12.9 mIU/10(7) spermatozoa) compared to the standard swim-up (80.6 +/- 4.9 mIU/10(7) spermatozoa). These results indicate that HFF components may influence sperm physiology and enhance sperm fertilizing capacity by activating sperm proteinase systems.

引用

页码：975 / 980

页数：6

共 30 条

[1]

Berger T., Marrs R.P., Sato M., Kletzky O.A., Human sperm penetration of zona-free hamster ova, Am. J. Obstet. Gynecol, 145, (1983)

[2]

Blumenfeld Z., Nahhas F., Pretreatment of sperm with human follicular fluid for borderline male infertility, Fertil. Steril, 51, pp. 863-868, (1989)

[3]

Bronson R., Cooper G., Rosenfeld D., Ability of antibody- bound human sperm to penetrate zona-free hamster ova in vitro, Fertil. Steril, 36, pp. 778-783, (1981)

[4]

Draho Rad J., Cechova D., Tesarik J., Activation of proacrosin by a locally produced component of human follicular fluid, J. Reprod. Fertil, 83, pp. 599-603, (1988)

[5]

Dravland J.E., Llanos M.N., Munn R.J., Meizel S., Evidence for the involvement of a trypsinlike enzyme in the membrane events of the hamster sperm acrosome reaction, J. Exp. Zool, 232, pp. 117-128, (1984)

[6]

Garbers D.L., Molecular basis of signalling in the spermatozoon, J. Androl, 10, pp. 99-107, (1989)

[7]

Goodpasture J.C., Polakoski K.L., Zaneveld L.J.D., Acrosin, proacrosin and acrosin inhibitor of human spermatozoa: Extraction, quantitation and stability, J. Androl, 1, pp. 16-27, (1979)

[8]

Lenz R.W., Ax R.L., Grimek H.J., First N.L., Proteoglycan from bovine follicular fluid enhances an acrosome reaction in bovine spermatozoa, Biochem. Biophys. Res. Commun, 106, pp. 1092-1098, (1982)

[9]

Lenz R.W., Beilin M.E., Ax R.L., Rabbit spermatozoa undergo an acrosome reaction in the presence of glycosaminoglycans, Gamete Res, 174, pp. 285-302, (1983)

[10]

Meizel S., Molecules that initiate or help stimulate the acrosome reaction by their interaction with the mammalian sperm surface, Am. J. Anat, 174, pp. 285-302, (1985)

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