HYPOTHALAMIC CATHEPSIN-D - ASSAY AND ISOENZYME COMPOSITION

被引：10

作者：

AKOPYAN, TN ^{[1
]}

BARCHUDARYAN, NA ^{[1
]}

KARABASHYAN, LV ^{[1
]}

ARUTUNYAN, AA ^{[1
]}

LAJTHA, A ^{[1
]}

GALOYAN, AA ^{[1
]}

机构：

[1] CTR NEUROCHEM,WARDS ISL,NY

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1979年 / 4卷 / 5-6期

关键词：

acid proteinases; cathepsin D; endopeptidase assay;

D O I：

10.1002/jnr.490040505

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A sensitive and convenient method of endopeptidase assay using as substrate globin modified with pyridoxal‐5‐phosphate was used for determination of acid proteinases in bovine hypothalamus separated by isoelectric focusing. The soluble protein fraction of hypothalamus upon elution from Sephadex gave five peaks of proteinase activity at pH 3.2. The properties indicate that these peaks of endopeptidase activity are isoenzyme forms of cathepsin D. Copyright © 1979 Alan R. Liss, Inc.

引用

页码：365 / 370

页数：6

共 19 条

[1] ACID PROTEINASE OF HYPOTHALAMUS PURIFICATION, SOME PROPERTIES, AND ACTION ON SOMATOSTATIN AND SUBSTANCE-P
AKOPYAN, TN
ARUTUNYAN, AA
LAJTHA, A
GALOYAN, AA
[J]. NEUROCHEMICAL RESEARCH, 1978, 3 (01) : 89 - 99
[2] GEL-FILTRATION BEHAVIOUR OF PROTEINS RELATED TO THEIR MOLECULAR WEIGHTS OVER A WIDE RANGE
ANDREWS, P
[J]. BIOCHEMICAL JOURNAL, 1965, 96 (03) : 595 - &
[3] CATHEPSIN-D - PURIFICATION OF ISOENZYMES FROM HUMAN AND CHICKEN LIVER
BARRETT, AJ
[J]. BIOCHEMICAL JOURNAL, 1970, 117 (03) : 601 - &
[4] BARRETT AJ, 1975, PROTEASES BIOL CONTR, V2, P467
[5] CUNNINGHAM M, 1976, J BIOL CHEM, V251, P4528
[6] DIRECT EVIDENCE OF IMPORTANCE OF LYSOSOMES IN DEGRADATION OF INTRACELLULAR PROTEINS
DEAN, RT
[J]. NATURE, 1975, 257 (5525) : 414 - 416
[7] IMMUNOINHIBITION OF INTRACELLULAR PROTEIN DIGESTION IN MACROPHAGES
DINGLE, JT
POOLE, AR
LAZARUS, GS
BARRETT, AJ
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1973, 137 (05) : 1124 - 1141
[8] INHIBITION OF RENIN-ANGIOTENSINOGEN REACTION BY PEPSTATIN
GROSS, F
LAZAR, J
ORTH, H
[J]. SCIENCE, 1972, 175 (4022) : 656 - &
[9] SEPARATION OF CATHEPSINS A AND D OF SKELETAL MUSCLE
IODICE, AA
LEONG, V
WEINSTOC.IM
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1966, 117 (03) : 477 - &
[10] CRYSTALLIZATION OF CATHEPSIN-D
KAZAKOVA, OV
OREKHOVICH, VN
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 72 (02) : 747 - 752

← 1 2 →