A NEW LUCIFERASE PROMOTER INSERTION VECTOR FOR THE ANALYSIS OF WEAK TRANSCRIPTIONAL ACTIVITIES

被引：24

作者：

DEMARTIN, R ^{[1
]}

STRASSWIMMER, J ^{[1
]}

PHILIPSON, L ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,W-6900 HEIDELBERG,GERMANY

来源：

GENE | 1993年 / 124卷 / 01期

关键词：

RECOMBINANT DNA; NEOMYCIN RESISTANCE; TRANSCRIPTION TERMINATION; VECTOR CONSTRUCTION; GENE REGULATION; MURINE GAS-I; C-MOS ONCOGENE;

D O I：

10.1016/0378-1119(93)90776-Y

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A new luciferase-encoding expression vector was generated by inserting the strong transcription termination signal from the mouse c-mos oncogene upstream from a multiple cloning site. This construct significantly reduced background transcription in NIH3T3 cells and has proven useful in the study of a weak promoter from the murine growth-arrest-specific gene gas-1.

引用

页码：137 / 138

页数：2

共 7 条

[1] HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].