DETERMINATION OF BACTERIOCIN-ENCODING PLASMIDS OF PEDIOCOCCUS-ACIDILACTICI STRAINS BY SOUTHERN HYBRIDIZATION

被引：10

作者：

BHUNIA, AK ^{[1
]}

BHOWMIK, TK ^{[1
]}

JOHNSON, MG ^{[1
]}

机构：

[1] UNIV ARKANSAS,ARKANSAS BIOTECHNOL CTR,FAYETTEVILLE,AR

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 18卷 / 03期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00836.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A 3.5 kb (2.3 MDa) plasmid probe (pRBI) containing the pap gene (pediocin AcH production) was used in Southern hybridization tests to determine the presence or absence of a plasmid-encoded pediocin gene in various isolates of Pediococcus. This probe hybridized with a 9.4 kb (6.2 MDa) plasmid found present in all pediocin-producing Pediococcus acidilactici strains tested. Conversely, this probe showed no reactions with Fed. acidilactici RS2-1, AB6-1 and PC, strains which did not produce pediocin and lacked the 9.4 kb (6.2 MDa) plasmid. This probe also did not hybridize with the plasmids from a bacteriocin producing Ped. pentosaceus strain.

引用

页码：168 / 170

页数：3

共 12 条

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