METABOLIC PATHWAYS IN EHRLICH ASCITES TUMOR-CELLS RECOVERING FROM A LOW BIOENERGETIC STATUS

Ehrlich ascites tumor cells were found to be in a low bioenergetic status, as evaluated by acridine orange uptake and ATP content, when resuspended in a glucose medium shortly after removal from the animal. Dye uptake as well as ATP content then increased for about 2 h at room temperature. This effect was only slightly inhibited by oligomycin. Cells resuspended in a glucose-free medium initially showed high dye uptake and ATP level, which were stable over time: in this case oligomycin caused a drop in both dye uptake and ATP level. The above findings, which are indicative of a marked Crabtree effect in Ehrlich ascites tumor tells, means that it is unlikely that limiting ADP and P-i play an important role in the glucose-induced inhibition of oxidative phosphorylation in this system.